LOCUS       40924_23879       12876 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Gateway binary vector pGWB761 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12876)
  AUTHORS   Tanaka Y, Nakamura S, Kawamukai M, Koizumi N, Nakagawa T.
  TITLE     Development of a series of gateway binary vectors possessing a 
            tunicamycin resistance gene as a marker for the transformation of 
            Arabidopsis thaliana
  JOURNAL   Biosci. Biotechnol. Biochem. 75 (4), 804-807 (2011)
  PUBMED    21512216
REFERENCE   2  (bases 1 to 12876)
  AUTHORS   Tanaka Y, Nakagawa T.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2011) Contact:Tsuyoshi Nakagawa Shimane 
            University, Center for Integrated Research in Science; 1060 
            Nishikawatsu, Matsue, Shimane 690-8504, Japan
REFERENCE   3  (bases 1 to 12876)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12876)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci. 
            Biotechnol. Biochem."; date: "2011"; volume: "75"; issue: "4"; 
            pages: "804-807"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-JAN-2011) Contact:Tsuyoshi Nakagawa Shimane University, Center 
            for Integrated Research in Science; 1060 Nishikawatsu, Matsue, 
            Shimane 690-8504, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     constructed using pPZP221.
FEATURES             Location/Qualifiers
     source          1..12876
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(54..78)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             1178..1888
                     /codon_start=1
                     /label=TagRFP
                     /note="monomeric derivative of red fluorescent protein from
                     Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     protein_bind    1898..2022
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        2059..2089
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             2143..2799
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             3144..3446
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(3490..3614)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      3640..3892
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3926..3942)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3950..3966
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3974..4004)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4019..4040)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     terminator      complement(4076..4328)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4366..5661)
                     /codon_start=1
                     /gene="gpt"
                     /product="UDP-N-acetylglucosamine: dolichol phosphate 
                     N-acetylglucosamine-1-P transferase"
                     /label=gpt
                     /protein_id="BAJ61303.1"
                     /translation="MAARKRASSISIPNKPDPSEPNSAPSEQKMTRKTVSASGEEFRLA
                     PPKLGVIFVISTLLCSLYLYLLCFHYKVDNELKRSILINAGLSLVGFFVTLKMIPVTAR
                     YVLRRNMFGFDINKRGTPQGDIKVPESLGIVVGIVFLIVAIIFQYFNFTEDSNWLVEYN
                     AALASICFMILLGFVDDVLDVPWRVKLVLPSFATLPLLMAYAGHTTIVIPKPLVAYIGL
                     EVLNLGRIYKLYMGLLAVFCTNSINIHAGLNGLEIGQTVVIAAAILIHNVMQIGASVDP
                     EYHQAHAFSIFLTQPLMATSLAMLAYNWYPSSVFVGDTYTVFAGMTMAVVGILGHFSET
                     LLIFFLPQVLNLLLSLPQLAGIVKCPRHRLPRYDPATGLLTGTKDGTLVNVYLRLFGPK
                     SEKSLCIHLLVFQALACAFCFILRHFLAGWYK"
     gene            complement(4366..5661)
                     /gene="gpt"
                     /label=gpt
     promoter        complement(5713..5892)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    complement(6398..6422)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6943..7731
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7980..8568
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(8754..8894)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(9238..9432)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9501..10571)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11003..11629)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag aatgagcatg gtgtctaagg gcgaagagct
     1201 gattaaggag aacatgcaca tgaagctgta catggagggc accgtgaaca accaccactt
     1261 caagtgcaca tccgagggcg aaggcaagcc ctacgagggc acccagacca tgagaatcaa
     1321 ggtggtcgag ggcggccctc tccccttcgc cttcgacatc ctggctacca gcttcatgta
     1381 cggcagcaga accttcatca accacaccca gggcatcccc gacttcttta agcagtcctt
     1441 ccctgagggc ttcacatggg agagagtcac cacatacgaa gacgggggcg tgctgaccgc
     1501 tacccaggac accagcctcc aggacggctg cctcatctac aacgtcaaga tcagaggggt
     1561 gaacttccca tccaacggcc ctgtgatgca gaagaaaaca ctcggctggg aggccaacac
     1621 cgagatgctg taccccgctg acggcggcct ggaaggcaga agcgacatgg ccctgaagct
     1681 cgtgggcggg ggccacctga tctgcaactt caagaccaca tacagatcca agaaacccgc
     1741 taagaacctc aagatgcccg gcgtctacta tgtggaccac agactggaaa gaatcaagga
     1801 ggccgacaaa gagacctacg tcgagcagca cgaggtggct gtggccagat actgcgacct
     1861 ccctagcaaa ctggggcaca aacttaatgc tgttatcaca agtttgtaca aaaaagctga
     1921 acgagaaacg taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca
     1981 gactacataa tactgtaaaa cacaacatat ccagtcacta tggcggcccc tcgaggggcc
     2041 gcattaggca ccccaggctt tacactttat gcttccggct cgtataatgt gtggattttg
     2101 agttaggatc cgtcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact
     2161 ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag
     2221 tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag
     2281 accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg
     2341 atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat
     2401 agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg
     2461 agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt
     2521 tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca
     2581 gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc
     2641 ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg
     2701 ctggcgattc aggttcatca tgccgtttgt gatggcttcc atgtcggcag aatgcttaat
     2761 gaattacaac agtactgcga tgagtggcag ggcggggcgt aaacgcgtgg atccggctta
     2821 ctaaaagcca gataacagta tgcgtatttg cgcgctgatt tttgcggtat aagaatatat
     2881 actgatatgt atacccgaag tatgtcaaaa agaggtatgc tatgaagcag cgtattacag
     2941 tgacagttga cagcgacagc tatcagttgc tcaaggcata tatgatgtca atatctccgg
     3001 tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc
     3061 ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc
     3121 tgacgagaac aggggctggt gaaatgcagt ttaaggttta cacctataaa agagagagcc
     3181 gttatcgtct gtttgtggat gtacagagtg atattattga cacgcccggg cgacggatgg
     3241 tgatccccct ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg
     3301 tggtgcatat cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgccgg
     3361 tctccgttat cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg
     3421 ccattaacct gatgttctgg ggaatataaa tgtcaggctc ccttatacac agccagtctg
     3481 caggtcgacc atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta
     3541 tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg ttcagctttc
     3601 ttgtacaaag tggtgataac ctagagctcg aatttccccg atcgttcaaa catttggcaa
     3661 taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg
     3721 ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg
     3781 gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag
     3841 cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tcgggaattg
     3901 gttccggaac caattcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     3961 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     4021 gagtgagcta actcacatta ggctgaatta ggcgcgccta tttctattaa ttcccgatct
     4081 agtaacatag atgacaccgc gcgcgataat ttatcctagt ttgcgcgcta tattttgttt
     4141 tctatcgcgt attaaatgta taattgcggg actctaatca taaaaaccca tctcataaat
     4201 aacgtcatgc attacatgtt aattattaca tgcttaacgt aattcaacag aaattatatg
     4261 ataatcatcg caagaccggc aacaggattc aatcttaaga aactttattg ccaaatgttt
     4321 gaacgatcgg ggaaattcgc ctcgagtgac ttctctgatt gcagactatt tataccaacc
     4381 agcaagaaag tgtcgaagta tgaagcagaa ggcgcaggct agagcctgga aaacgagaag
     4441 atggatgcaa agagactttt ctgatttagg accaaacaac ctcaagtaaa cattgacgag
     4501 tgtcccatct tttgtgccgg tcagtaatcc ggtagcagga tcataccttg ggagacgatg
     4561 gcgcggacat ttcacaatcc cagcaagctg aggaagcgat aatagaaggt tcagaacttg
     4621 aggaagaaag aagatgagga gggtttcact gaagtgaccc agaataccaa caactgccat
     4681 agtcattccg gcgaagactg tgtaagtgtc tccaacaaaa actgaagaag gataccaatt
     4741 gtaagcaagc attgccaagg atgtggccat cagcggttga gtaagaaata tcgagaaggc
     4801 atgagcttgg tgatactcgg gatcaacaga tgctccgatt tgcataacgt tgtgtatcag
     4861 gatagcagcg gcaataacaa ctgtttgacc gatttcaagg ccattcagac cagcgtggat
     4921 gttaatagag tttgtacaaa agacagcaag tagccccata tataacttat atatccttcc
     4981 tagattaagc acttccaagc caatataagc aacaagaggt tttggtataa caatagttgt
     5041 atgaccagca taagccatca aaagtggaag ggtagcaaaa gatggcagga caagttttac
     5101 tctccaaggc acatcaagga catcatctac aaatccaagc aaaatcatga agcaaataga
     5161 tgctagtgct gcattatact ccacaagcca attagaatct tcagtgaagt taaaatactg
     5221 aaatattatc gccacgatca aaaagacaat accaacaaca atacccaatg actctggcac
     5281 tttaatatct ccttgaggcg tgcctctttt gttgatatca aaaccaaaca tgttacgtct
     5341 cagaacgtat ctggcagtaa caggaatcat tttgagagtg acgaaaaacc caaccaagct
     5401 aagccccgcg ttaattagaa tcgatcgctt gagttcatta tcaaccttgt agtgaaaaca
     5461 gagaaggtat agatagagcg aacagagaag agtagagatc acgaagatca ctcctaattt
     5521 cggcggcgcg agacgaaatt cctcaccgga agcagaaacc gtctttcggg tcatcttctg
     5581 ctccgatggc gctgagttgg gttccgacgg gtccggtttg ttgggaattg agatcgatga
     5641 agctctctta cgagctgcca tatcttattg ccccccggga tcagatctgg attgagagtg
     5701 aatatgagac tctaattgga taccgagggg aatttatgga acgtcagtgg agcatttttg
     5761 acaagaaata tttgctagct gatagtgacc ttaggcgact tttgaacgcg caataatggt
     5821 ttctgacgta tgtgcttagc tcattaaact ccagaaaccc gcggctgagt ggctccttca
     5881 acgttgcggt tctgtcagtt ccaaacgtaa aacggcttgt cccgcgtcat cggcgggggt
     5941 cataacgtga ctcccttaat tctccgctca tgatcttgat cccctgcgcc atcagatcct
     6001 tggcggcaag aaagccatcc agtttacttt gcagggcttc ccaaccttac cagagggcgc
     6061 cccagctggc aattccggtt cgcttgctgt ccataaaacc gcccagtcta gctatcgcca
     6121 tgtaagccca ctgcaagcta cctgctttct ctttgcgctt gcgttttccc ttgtccagat
     6181 agcccagtag ctgacattca tccggggtca gcaccgtttc tgcggactgg ctttctacgt
     6241 gttccgcttc ctttagcagc ccttgcgccc tgagtgcttg cggcagcgtg aagctgatcc
     6301 gtcgagatta atagaaataa attcagccta attcggcgtt aattcagtac attaaaaacg
     6361 tccgcaatgt gttattaagt tgtctaagcg tcaatttgtt tacaccacaa tatatcctgc
     6421 caccagccag ccaacagctc cccgaccggc agctcggcac aaaatcacca ctcgatacag
     6481 gcagcccatc agtccgggac ggcgtcagcg ggagagccgt tgtaaggcgg cagactttgc
     6541 tcatgttacc gatgctattc ggaagaacgg caactaagct gccgggtttg aaacacggat
     6601 gatctcgcgg agggtagcat gttgattgta acgatgacag agcgttgctg cctgtgatca
     6661 attcgggcac gaacccagtg gacataagcc tgttcggttc gtaagctgta atgcaagtag
     6721 cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca
     6781 gtggcggttt tcatggcttg ttatgactgt ttttttgggg tacagtctat gcctcgggca
     6841 tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat
     6901 gttacgcagc agggcagtcg ccctaaaaca aagttaaaca tcatggggga agcggtgatc
     6961 gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg
     7021 acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagt
     7081 gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg
     7141 atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta
     7201 gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa
     7261 ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg
     7321 atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta
     7381 ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta
     7441 aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta
     7501 gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat
     7561 gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa
     7621 gctagacagg cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg
     7681 gaagaatttg tccactacgt gaaaggcgag atcaccaagg tagtcggcaa ataatgtcta
     7741 gctagaaatt cgttcaagcc gacgccgctt cgcggcgcgg cttaactcaa gcgttagatg
     7801 cactaagcac ataattgctc acagccaaac tatcaggtca agtctgcttt tattattttt
     7861 aagcgtgcat aataagccct acacaaattg ggagatatat catgcatgac caaaatccct
     7921 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     7981 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     8041 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     8101 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     8161 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     8221 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     8281 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     8341 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     8401 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     8461 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     8521 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     8581 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     8641 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     8701 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
     8761 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt
     8821 acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact
     8881 gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     8941 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     9001 ggttttcacc gtcatcaccg aaacgcgcga ggcagggtgc cttgatgtgg gcgccggcgg
     9061 tcgagtggcg acggcgcggc ttgtccgcgc cctggtagat tgcctggccg taggccagcc
     9121 atttttgagc ggccagcggc cgcgataggc cgacgcgaag cggcggggcg tagggagcgc
     9181 agcgaccgaa gggtaggcgc tttttgcagc tcttcggctg tgcgctggcc agacagttat
     9241 gcacaggcca ggcgggtttt aagagtttta ataagtttta aagagtttta ggcggaaaaa
     9301 tcgccttttt tctcttttat atcagtcact tacatgtgtg accggttccc aatgtacggc
     9361 tttgggttcc caatgtacgg gttccggttc ccaatgtacg gctttgggtt cccaatgtac
     9421 gtgctatcca caggaaagag accttttcga cctttttccc ctgctagggc aatttgccct
     9481 agcatctgct ccgtacatta ggaaccggcg gatgcttcgc cctcgatcag gttgcggtag
     9541 cgcatgacta ggatcgggcc agcctgcccc gcctcctcct tcaaatcgta ctccggcagg
     9601 tcatttgacc cgatcagctt gcgcacggtg aaacagaact tcttgaactc tccggcgctg
     9661 ccactgcgtt cgtagatcgt cttgaacaac catctggctt ctgccttgcc tgcggcgcgg
     9721 cgtgccaggc ggtagagaaa acggccgatg ccgggatcga tcaaaaagta atcggggtga
     9781 accgtcagca cgtccgggtt cttgccttct gtgatctcgc ggtacatcca atcagctagc
     9841 tcgatctcga tgtactccgg ccgcccggtt tcgctcttta cgatcttgta gcggctaatc
     9901 aaggcttcac cctcggatac cgtcaccagg cggccgttct tggccttctt cgtacgctgc
     9961 atggcaacgt gcgtggtgtt taaccgaatg caggtttcta ccaggtcgtc tttctgcttt
    10021 ccgccatcgg ctcgccggca gaacttgagt acgtccgcaa cgtgtggacg gaacacgcgg
    10081 ccgggcttgt ctcccttccc ttcccggtat cggttcatgg attcggttag atgggaaacc
    10141 gccatcagta ccaggtcgta atcccacaca ctggccatgc cggccggccc tgcggaaacc
    10201 tctacgtgcc cgtctggaag ctcgtagcgg atcacctcgc cagctcgtcg gtcacgcttc
    10261 gacagacgga aaacggccac gtccatgatg ctgcgactat cgcgggtgcc cacgtcatag
    10321 agcatcggaa cgaaaaaatc tggttgctcg tcgcccttgg gcggcttcct aatcgacggc
    10381 gcaccggctg ccggcggttg ccgggattct ttgcggattc gatcagcggc cgcttgccac
    10441 gattcaccgg ggcgtgcttc tgcctcgatg cgttgccgct gggcggcctg cgcggccttc
    10501 aacttctcca ccaggtcatc acccagcgcc gcgccgattt gtaccgggcc ggatggtttg
    10561 cgaccgctca cgccgattcc tcgggcttgg gggttccagt gccattgcag ggccggcaga
    10621 caacccagcc gcttacgcct ggccaaccgc ccgttcctcc acacatgggg cattccacgg
    10681 cgtcggtgcc tggttgttct tgattttcca tgccgcctcc tttagccgct aaaattcatc
    10741 tactcattta ttcatttgct catttactct ggtagctgcg cgatgtattc agatagcagc
    10801 tcggtaatgg tcttgccttg gcgtaccgcg tacatcttca gcttggtgtg atcctccgcc
    10861 ggcaactgaa agttgacccg cttcatggct ggcgtgtctg ccaggctggc caacgttgca
    10921 gccttgctgc tgcgtgcgct cggacggccg gcacttagcg tgtttgtgct tttgctcatt
    10981 ttctctttac ctcattaact caaatgagtt ttgatttaat ttcagcggcc agcgcctgga
    11041 cctcgcgggc agcgtcgccc tcgggttctg attcaagaac ggttgtgccg gcggcggcag
    11101 tgcctgggta gctcacgcgc tgcgtgatac gggactcaag aatgggcagc tcgtacccgg
    11161 ccagcgcctc ggcaacctca ccgccgatgc gcgtgccttt gatcgcccgc gacacgacaa
    11221 aggccgcttg tagccttcca tccgtgacct caatgcgctg cttaaccagc tccaccaggt
    11281 cggcggtggc ccatatgtcg taagggcttg gctgcaccgg aatcagcacg aagtcggctg
    11341 ccttgatcgc ggacacagcc aagtccgccg cctggggcgc tccgtcgatc actacgaagt
    11401 cgcgccggcc gatggccttc acgtcgcggt caatcgtcgg gcggtcgatg ccgacaacgg
    11461 ttagcggttg atcttcccgc acggccgccc aatcgcgggc actgccctgg ggatcggaat
    11521 cgactaacag aacatcggcc ccggcgagtt gcagggcgcg ggctagatgg gttgcgatgg
    11581 tcgtcttgcc tgacccgcct ttctggttaa gtacagcgat aaccttcatg cgttcccctt
    11641 gcgtatttgt ttatttactc atcgcatcat atacgcagcg accgcatgac gcaagctgtt
    11701 ttactcaaat acacatcacc tttttagacg gcggcgctcg gtttcttcag cggccaagct
    11761 ggccggccag gccgccagct tggcatcaga caaaccggcc aggatttcat gcagccgcac
    11821 ggttgagacg tgcgcgggcg gctcgaacac gtacccggcc gcgatcatct ccgcctcgat
    11881 ctcttcggta atgaaaaacg gttcgtcctg gccgtcctgg tgcggtttca tgcttgttcc
    11941 tcttggcgtt cattctcggc ggccgccagg gcgtcggcct cggtcaatgc gtcctcacgg
    12001 aaggcaccgc gccgcctggc ctcggtgggc gtcacttcct cgctgcgctc aagtgcgcgg
    12061 tacagggtcg agcgatgcac gccaagcagt gcagccgcct ctttcacggt gcggccttcc
    12121 tggtcgatca gctcgcgggc gtgcgcgatc tgtgccgggg tgagggtagg gcgggggcca
    12181 aacttcacgc ctcgggcctt ggcggcctcg cgcccgctcc gggtgcggtc gatgattagg
    12241 gaacgctcga actcggcaat gccggcgaac acggtcaaca ccatgcggcc ggccggcgtg
    12301 gtggtgtcgg cccacggctc tgccaggcta cgcaggcccg cgccggcctc ctggatgcgc
    12361 tcggcaatgt ccagtaggtc gcgggtgctg cgggccaggc ggtctagcct ggtcactgtc
    12421 acaacgtcgc cagggcgtag gtggtcaagc atcctggcca gctccgggcg gtcgcgcctg
    12481 gtgccggtga tcttctcgga aaacagcttg gtgcagccgg ccgcgtgcag ttcggcccgt
    12541 tggttggtca agtcctggtc gtcggtgctg acgcgggcat agcccagcag gccagcggcg
    12601 gcgctcttgt tcatggcgta atgtctccgg ttctagtcgc aagtattcta ctttatgcga
    12661 ctaaaacacg cgacaagaaa acgccaggaa aagggcaggg cggcagcctg tcgcgtaact
    12721 taggacttgt gcgacatgtc gttttcagaa gacggctgca ctgaacgtca gaagccgact
    12781 gcactatagc agcggagggg ttggatcaaa gtactttgat cccgagggga accctgtggt
    12841 tggcatgcac atacaaatgg acgaacggat aaacct
//
